Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effect of THADA knockdown on Huh7 cells

ABSTRACT: We use lentivirus carrying shRNA for THADA to evaluate the efect of THADA knockdown in Huh7 cells. The pGIPZ shRNA plasmid specific to human THADA (clone ID V3LHS_318037 Open Biosystems, Inc.) or the pGIPZ non-silencing shRNAmir lentiviral plasmid (as a control) was cotransfected with the pPACKH1 plasmid mix in HEK 293 cells. Stable clones of Huh7 cells with THADA knockdown were routinely maintained in a medium containing of puromycin (500ng/ml)

ORGANISM(S): Homo sapiens

SUBMITTER: Takafumi Yoshida

PROVIDER: E-GEOD-65233 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Inhibition of AK4 expression increases drug sensitivity and elevates mitochondrial ATP synthesis

Project description:Adenylate kinase 4 (AK4) is localized in the mitochondrial matrix, and is believed to be involved in stress, drug resistance, the malignant transformation of cancer, and ATP regulation. We produced AK4 knockdown HeLa cells by using shRNA and used these to analyze resistance, and found that sensitivity to hypoxia and drugs increased. 3 samples of HeLa cells haboring AK4 shRNA plasmids and 4 samples of HeLa cells haboring control shRNA plasmid.

2016-03-31 | E-GEOD-61843 | biostudies-arrayexpress

The effect of THADA knockdown on Huh7 cells

Project description:We use lentivirus carrying shRNA for THADA to evaluate the efect of THADA knockdown in Huh7 cells.

2015-08-01 | GSE65233 | GEO

Illumina sequencing analysis of LINC00998 knockdown and control in Huh7 cell line

Project description:shRNA knockdown against LINC00998 in Huh7 cells followed by RNA-seq. LINC00998 is also known as SMIM30 or ENSG00000214194.

2019-04-04 | GSE129277 | GEO

Expression data comparing Huh7 cells transduced with shRNA CD24 (knockdown) or non-target control

Project description:To identify CD24 signaling pathway Affymetrix Human Genome U133 Plus GeneChip 2.0 shRNA CD24 (knockdown) or non-target control (NTC) was stably transduced into Huh7 HCC cells by lentiviral approach

2012-01-01 | E-GEOD-28057 | biostudies-arrayexpress

RNAseq analysis of Huh7 cells upon stable-knockdown of L1 elements

Project description:L1 elements are repeat elements known to get activated during hepatocarcinogenesis. We have knocked-down expression of L1 elements using shRNA targeting L1-ORF1 in Huh7 cell line and a control non-targeting cell line was also generated. Influence of L1 knockdown on overall cellular transcritome was then analysed by RNAseq followed by pathway analysis. Overall, a crosstalk between L1 elements and TGFbeta signaling pathway is observed.

2023-01-21 | GSE126615 | GEO

Investigation of downstream genes regulated by ACOT12 in HCC cells [Huh7]

Project description:Huh7 cell, a human hepatocellular carcinoma (HCC) cell line, was stably overexpressed with scramble shRNA or shRNA against ACOT12. These cells were then used for gene expression profiles analysis.

2019-01-01 | GSE111760 | GEO

Expression profile in Huh7 cell treated with KRT19 shRNA

Project description:We aimed to investigate the involvement of genes in the down-regulation of KRT19 in Huh7 cell lines. Stably knocked down KRT-19 in Huh7, the corresponding empty plasmid of PLL3.7 treated control cells were applied to extract the total RNA from three samples in each group, and were amplified and transcribed into fluorescent complementary DNA (cDNA). Labeled samples were hybridized to the Human Roche NimbleGen mRNA microarray (Roche, Basel, Switzerland).

2015-01-19 | E-MTAB-2559 | biostudies-arrayexpress

RNA-seq data for EphA2 knockdown in Huh7 cells

Project description:RNA-seq data for EphA2 knockdown (KD) in Huh7 cells

2021-02-25 | GSE141880 | GEO

RNA-seq data for ABL1 knockdown in Huh7 cells

Project description:RNA-seq data for ABL1 knockdown (KD) in Huh7 cells

2021-06-30 | GSE133294 | GEO

KCa3.1 depletion effect on hepatocellular carcinoma cell line Huh7

Project description:Analysis of Huh7 hepatocellular carcinoma (HCC) cells depleted for potassium channel KCa3.1, the intermediate conductance calcium-activated potassium channel. Result provide insight into the role of KCa3.1 in the carcinogenesis of HCC. We used microarrays to detail the gene expression differences between KCa3.1 knockdown and negative control in Huh7 HCC cells.

2018-06-23 | GSE116183 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data