RNA-seq Analysis of MoRFX1-dependent Genes in Magnaporthe oryzae 70-15

ABSTRACT: Purpose: The goals of this study are to find out the genes regulated directly or indirectly by the regulatory factor X protein MoRfx1 by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMorfx1 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). RNA-sequencing libraries were constructed using NEBNext RNA sample preparation kit (NEB). Samples were sequenced in a 1 x 100 nt way on Illumina Hiseq2500 instrument using TruSeq PE Cluster Kit v3 - cBot - HS (Illumina) and TruSeq SBS Kit v3-HS (Illumina).The M. oryzae genome database (MG8) (www.broadinstitute.org) was used as a reference gene database. Genome-wide transcript levels of gene

ORGANISM(S): Magnaporthe oryzae

SUBMITTER: Jian Ping Lu 

PROVIDER: E-GEOD-65296 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress