The Role of Macrophages in the Development of Human Renal Allograft Fibrosis in the First Year after Transplantation

ABSTRACT: The aim of this study was to investigate the role of infiltrating macrophages in renal allograft fibrosis. Forty-six protocol renal allograft biopsies obtained one-year after transplantation were stained with Sirius Red to quantify fibrosis and double stained with CD68 and CD206 to identify the proportion of alternately activated (M2) macrophages. 23 protocol biopsies obtained 12 months post transplant were analyzed for gene expression by microarray, which was correlated with macrophage infiltration and the severity of fibrosis. Phenotypic analysis showed 92% of infiltrating macrophages exhibited an M2 phenotype with CD68+CD206+ dual staining. Gene microarrays demonstrated a distinct alloimmune response despite the lack of rejection and inflammatory infiltrate with upregulation of interfe

ORGANISM(S): Homo sapiens

SUBMITTER: Philip O'Connell 

PROVIDER: E-GEOD-65326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress