Functional impact of collagens on the activity directed by the promoter of the a5 integrin subunit gene in corneal epithelial cells

ABSTRACT: Important remodeling of the extracellular matrix is a hallmark of corneal wound healing. Besides the massive secretion of fibronectin (FN) that characterizes the early step of that process, alterations in the secretion of collagens also occurs as part of this wound healingresponse. In this study, we examined whether expression of the gene encoding the M-NM-15 subunit from the FN binding integrin M-NM-15M-NM-21 changes as corneal epithelial cells (CECs) are cultured in the presence of collagens. Responsiveness of the M-NM-15 gene toward collagen was determined by transfection of recombinant plasmids bearing the CAT reporter gene under the control of various segments from the human M-NM-15 promoter into primary cultured rabbit (RCECs) and human (HCECs) CECs cultured on BSA or on collagens. E

ORGANISM(S): Homo sapiens

SUBMITTER: Karine Zaniolo 

PROVIDER: E-GEOD-65421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress