Real-time quantitative PCR analysis of human ependymal and ventral horn regions in adult control spinal cord

ABSTRACT: Adult human ependymal and ventral horn regions were obtained from postmortem frozen samples by Laser Capture Microdissection. Briefly, Cryostat 25 micron sections from were stained with toluidin blue and both regions microdissected and collected on eppendorf (n=4 for each region). Samples mRNA concentration and purity was assessed by electrophoresis (BioRad Experion HighSensitivity kit, USA). RQI values were lower than 6,5 in every case, so that purification was followed by 2 cycle amplification with a kit designed for highly degraded samples (ExpressArt® TRinucleotide mRNA Amplification Kit; #6299-A15, AmpTec, AMSBIO, UK). After amplification, mRNA concentration and purity was assessed both by electrophoresis (BioRad Experion StSens kit, USA) and by spectrophotometry (Nanodrop, Thermo Sci

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Garcia-Ovejero 

PROVIDER: E-GEOD-65463 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress