Single cell analysis of macrophages exposed to beads coated with LPS from Salmonella enterica subsp. typhimurium

ABSTRACT: We present a detailed single cell analysis of the macrophage response to LPS from Salmonella enterica. By combining single cell transcriptional analysis, fluorescently labeled, LPS-coated beads, and cytometry we are able to distinguish the responses of macrophages that have internalized LPS-coated beads and those that have not. Analysis of 96 single macrophages that were either: left untreated, were exposed to but did not internalize uncoated beads, were exposed to and internalized uncoated beads, were exposed to but did not internalize LPS-coated beads, or were exposed to and did internalize LPS-coated beads.

ORGANISM(S): Mus musculus

SUBMITTER: Deborah Hung 

PROVIDER: E-GEOD-65529 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress