Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Single cell analysis of macrophages exposed to beads coated with LPS from Salmonella enterica subsp. typhimurium


ABSTRACT: We present a detailed single cell analysis of the macrophage response to LPS from Salmonella enterica. By combining single cell transcriptional analysis, fluorescently labeled, LPS-coated beads, and cytometry we are able to distinguish the responses of macrophages that have internalized LPS-coated beads and those that have not. Analysis of 96 single macrophages that were either: left untreated, were exposed to but did not internalize uncoated beads, were exposed to and internalized uncoated beads, were exposed to but did not internalize LPS-coated beads, or were exposed to and did internalize LPS-coated beads.

ORGANISM(S): Mus musculus

SUBMITTER: Deborah Hung 

PROVIDER: E-GEOD-65529 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Encounters between immune cells and invading bacteria ultimately determine the course of infection. These interactions are usually measured in populations of cells, masking cell-to-cell variation that may be important for infection outcome. To characterize the gene expression variation that underlies distinct infection outcomes and monitor infection phenotypes, we developed an experimental system that combines single-cell RNA-seq with fluorescent markers. Probing the responses of individual macr  ...[more]

Similar Datasets

2015-09-07 | GSE65529 | GEO
| PRJEB35804 | ENA
2007-09-29 | E-GEOD-935 | biostudies-arrayexpress
| PRJNA274281 | ENA
2010-12-07 | GSE19748 | GEO
2010-05-05 | E-GEOD-17761 | biostudies-arrayexpress
2008-06-17 | E-GEOD-8507 | biostudies-arrayexpress
2019-11-21 | GSE139457 | GEO
2024-09-30 | E-MTAB-13554 | biostudies-arrayexpress
2020-09-14 | PXD021332 | Pride