Similarities and Distinctions in Actions of Surface-Directed and Classic Androgen Receptor Antagonists

ABSTRACT: This study was undertaken to compare efficacy of MJC13 and the classic AR antagonist flutamide. Both compounds modulate endogenous AR target gene expression in Prostate cancer cells in a similar fashion despite different mechanisms of action. LNCaP cells were seeded within 6-well dishes at density of 1 × 100,000 within RPMI 1640 media supplemented with L-glutamine and 10% fetal bovine serum (FBS), day 2 the media replaced with RPMI 1640 media supplemented with L-glutamine and 10% charcoal-stripped FBS, day 3 the cells were treated with DMSO (control), 10nM DHT, 1μM Flutamide, 1μM Flutamide + 10nM DHT, 30μM MJC13, or 30μM MJC13 + 10nM DHT for 24 hours, after which total RNA was isolated.

ORGANISM(S): Homo sapiens

SUBMITTER: Paul Webb 

PROVIDER: E-GEOD-65562 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress