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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-Wide Transcription Responses to Synchrotron Microbeam Radiotherapy

ABSTRACT: We hypothesized that gene expression changes in EMT6.5 tumors after Microbeam Radiotherapy (MRT) are different from those seen after broad beam (BB) at 4-48 h postirradiation. Our findings demonstrate molecular differences in the tumor response to MRT vs. BB and provide insight into the underlying mechanisms of MRT and BB. Total RNA obtained from isolated EMT6.5 tumours irradiated by MRT or BB at 4, 24 or 48 hours post-irradiation compared to unirradiated control tumours.

ORGANISM(S): Mus musculus

SUBMITTER: Yuqing Yang

PROVIDER: E-GEOD-65625 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-Wide Transcription Responses to Synchrotron Microbeam Radiotherapy

Project description:We hypothesized that gene expression changes in EMT6.5 tumors after Microbeam Radiotherapy (MRT) are different from those seen after broad beam (BB) at 4-48 h postirradiation. Our findings demonstrate molecular differences in the tumor response to MRT vs. BB and provide insight into the underlying mechanisms of MRT and BB.

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Gene expression profiles of primary tumors with radiotherapy and lung metastases in mouse squamous cell carcinoma model.

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LET-dependent change in rice gene expression profile after heavy-ion beam irradiation [LET]

Project description:Linear energy transfer (LET) is an important factor affecting several aspects of the irradiation effect, e.g. cell survival and mutation frequency, making the heavy-ion beam an effective mutagen. To study the mechanisms behind LET-dependent effects, expression profiling was performed after heavy-ion beam irradiation of imbibed rice seeds. Array-based experiments at three time points (0.5, 1, 2 h after the irradiation) revealed that the number of up- or down-regulated genes was highest 2 h after irradiation. Array-based experiments with four different LETs at 2 h after irradiation identified LET-independent regulated genes that were up/down-regulated regardless of the value of LET; LET-dependently regulated genes, whose expression level increased with the rise of LET value, were also identified. Oryza sativa L. 'Nipponbare' seeds were imbibed for 3 days. The seeds were irradiated with 22.5 or 50 keV/Î¼m C-ion or 63 or 80 keV/Î¼m Ne-ion at a dose of 15 Gy. Gene expressions of irradiated and unirradiated embryos were measured at 2 hours after irradiation. Three independent experiments were performed at each time and LET.

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Normal human fibroblast cell line bystander effects by Carbon ion irradiation

Project description:We have recently investigated the response of irradiated and bystander fibroblasts to heavy ions in confluent cultures exposed randomly to broadbeam and selectively to precision microbeam that target a predefined fraction of cells with counted number of particle(s), respectively, and found the difference in the time course of apoptosis induction and p53 phosphorylation between irradiated and bystander cells [Hamada MR2008]. In the present investigation, to further scrutinize the underpinnings of their temporally distinct responses, we set out to examine the gene expression changes in irradiated and bystander fibroblasts at a genome-wide level with the microarray analysis under the same experimental condition. We demonstrate that the gene expression profiles of bystander cells are substantially different from those of heavy ion-irradiated cells. Keywords: bystander effect, hour-time course cell number dependency, irradiation-dose response 36 samples ; Broadbeam control A, Broadbeam control B, Broadbeam sham 2 h A, Broadbeam sham 2 h B, Broadbeam sham 6 h A, Broadbeam sham 6 h B, Broadbeam D 2 h A, Broadbeam D 2 h B, Broadbeam D 6 h A, Broadbeam D 6 h B, Broadbeam 0.1D 2 h A, Broadbeam 0.1D 2 h B, Broadbeam 0.1D 6 h A, Broadbeam 0.1D 6 h B, Broadbeam 0.01D 2 h A, Broadbeam 0.01D 2 h B, Broadbeam 0.01D 6 h A, Broadbeam 0.01D 6 h B, Microbeam Control A, Microbeam Control B, Microbeam sham 2 h A, Microbeam sham 2 h B, Microbeam sham 6 h A, Microbeam sham 6 h B, Microbeam 1c 2 h A, Microbeam 1c 2 h B, Microbeam 1c 6 h A, Microbeam 1c 6 h B, Microbeam 5c 2 h A, Microbeam 5c 2 h B, Microbeam 5c 6 h A, Microbeam 5c 6 h B, Microbeam 25c 2 h A, Microbeam 25c 2 h B, Microbeam 25c 6 h A, Microbeam 25c 6 h B.

2008-09-02 | E-GEOD-8993 | biostudies-arrayexpress

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