Target genes associated to siRNA mediated repression of Pea3 and Erm transcription factors in mammary cancerous cells

ABSTRACT: Pea3 and Erm are transcription factors belonging to the ETS family which are involved in tumorigenesis, particularly mammary oncogenesis. To elucidate the mechanism of Pea3/Erm induced tumorigenesis in mouse mammary cancerous MMT cells (ATCC CCL-51), we have down-regulated Pea3 or Erm expression in this cell line by using small interfering RNA and performed a microarray analysis using Applied Biosystems AB1700 technology to define target associated genes. Keywords: Comparative transcriptome hybridization, small interfering RNA, mouse mammary cancerous cells Mouse mammary cancerous MMT cells (ATCC CCL-51) have been transfected with a siRNA control, an erm-directed siRNA or an pea3-directed siRNA. MMT cells were also used to determine the modifications due to the transfection protocol. These

ORGANISM(S): Mus musculus

SUBMITTER: Anne Chotteau 

PROVIDER: E-GEOD-6588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress