Integrative analysis of RNA, translation and protein levels reveals distinct regulatory variation across humans

ABSTRACT: Elucidating the extent and consequences of genetic differences between humans is essential for understanding phenotypic diversity and personalized medicine. Although variation in RNA levels, transcription factor binding and chromatin have been explored, little is known about global variation in translation and its genetic determinants among humans. We used ribosome profiling, RNA sequencing, and mass spectrometry to perform an integrated analysis in lymphoblastoid cell lines from a diverse group of individuals. We find significant differences in RNA levels, translation, and protein abundance suggesting diverse mechanisms of personalized gene expression control. Combined analysis of RNA expression and ribosome occupancy improves the identification of individual protein level differences. F

ORGANISM(S): Homo sapiens

SUBMITTER: Can Cenik 

PROVIDER: E-GEOD-65912 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress