Transcription profiling of mouse megakaryocyte differentiation

ABSTRACT: Megakaryocyte (MK) differentiation is well described in morphologic terms but its molecular counterparts and the basis for platelet release are incompletely understood. We profiled mRNA expression in populations of primary mouse MKs representing successive differentiation stages. Genes associated with DNA replication are highly expressed in young MKs, in parallel with endomitosis. Intermediate stages are characterized by disproportionate expression of genes associated with the cytoskeleton, cell migration and G-protein signaling, whereas terminally mature MKs accumulate hemostatic factors, including many membrane proteins. We used these expression profiles to extract a reliable panel of molecular markers for MKs of early, intermediate or advanced differentiation, and establish its value us

ORGANISM(S): Mus musculus

SUBMITTER: Ramesh Shivdasani 

PROVIDER: E-GEOD-6593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress