Transcriptomic analysis of cultured corneal endothelial cells as a validation for their use in cell-replacement therapy

ABSTRACT: The corneal endothelium plays a primary role in maintaining corneal homeostasis and clarity, and must be surgically replaced with allogenic donor corneal endothelium in the event of visually significant dysfunction. However, a worldwide shortage of donor corneal tissue has led to a search for alternative sources of transplantable tissue. Cultured human corneal endothelial cells (HCEnC) have been shown to restore corneal clarity in experimental models of corneal endothelial dysfunction in animal models, but characterization of cultured HCEnC remains incomplete. To this end, we utilized next-generation RNA sequencing technology to compare the transcriptomic profile of ex vivo human corneal endothelium (evHCEnC) with that of primary HCEnC and HCEnC lines, and to determine the utility of cultu

ORGANISM(S): Homo sapiens

SUBMITTER: Anthony Aldave 

PROVIDER: E-GEOD-65991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress