Transcriptome changes in Fusarium verticillioides caused by mutation in the transporter-like gene FST1
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ABSTRACT:
ORGANISM(S): Fusarium verticillioides
SUBMITTER: Charles Woloshuk
PROVIDER: E-GEOD-66044 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:An gene-expression comparison of the wild type and strain delta-fst1 of Fusarium verticillioides grown on autoclaved maize kernels for six days was conducted.
2015-02-19 | GSE66044 | GEO
Project description:Fusarium verticillioides is a detrimental fungus that can contaminate maize grains with mycotoxins that are harmful to human and animal health. Breeding and growing resistant genotypes is one alternative to reduce contamination and subsequent production of mycotoxins by this fungus. However, little is known about the resistant mechanism relevant to breeding in this pathosystem. Therefore, our aim was to identify genes and metabolites that may be related to Fusarium ear rot resistance using resistant and susceptible maize inbreds. Kernels of the resistant inbred showed significantly reduced disease severity, and reduced levels of total fumonisin and ergosterol content compared with the susceptible one. Gene expression data were obtained from microarray hybridizations using F. verticillioides inoculated and non inoculated maize kernels. Differentially expressed sequences were identified and classified into 36 functional categories. Most of the differentially expressed genes were assigned to the categories “protein, RNA, DNA, stress, transport, signaling and cell metabolism”. These genes encode for PR proteins, detoxification and primary metabolism enzymes. Fungal inoculation did not produce considerable changes in gene expression and metabolites in the resistant L4637 inbred, probably due to a preformed or constitutive resistance mechanism. Defense-related genes were induced or repressed in kernels of the susceptible inbred L4674, responding specifically to the pathogen infection. The qRT-PCR in infected silks showed that glucanase, lipid transfer, xylanase inhibitor, PR1 and 26S proteosome transcripts had higher expression ratios in the susceptible line compared to the resistant one in response to fungal infection. Through this study, a global view of differential genes expressed and metabolites concentration during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize. Global view of differential genes expressed during resistance and susceptibility to F. verticillioides inoculation. Two maize inbred lines : one resistant (L4637) and one susceptible (L4674) to F. verticillioides infection. Two-condition experiment, Inoculated (I) vs. non-inoculated (NI) lines. Biological replicates: 3 . One replicate per array.
2013-08-17 | E-GEOD-40288 | biostudies-arrayexpress
Project description:Fusarium verticillioides is a detrimental fungus that can contaminate maize grains with mycotoxins that are harmful to human and animal health. Breeding and growing resistant genotypes is one alternative to reduce contamination and subsequent production of mycotoxins by this fungus. However, little is known about the resistant mechanism relevant to breeding in this pathosystem. Therefore, our aim was to identify genes and metabolites that may be related to Fusarium ear rot resistance using resistant and susceptible maize inbreds. Kernels of the resistant inbred showed significantly reduced disease severity, and reduced levels of total fumonisin and ergosterol content compared with the susceptible one. Gene expression data were obtained from microarray hybridizations using F. verticillioides inoculated and non inoculated maize kernels. Differentially expressed sequences were identified and classified into 36 functional categories. Most of the differentially expressed genes were assigned to the categories “protein, RNA, DNA, stress, transport, signaling and cell metabolism”. These genes encode for PR proteins, detoxification and primary metabolism enzymes. Fungal inoculation did not produce considerable changes in gene expression and metabolites in the resistant L4637 inbred, probably due to a preformed or constitutive resistance mechanism. Defense-related genes were induced or repressed in kernels of the susceptible inbred L4674, responding specifically to the pathogen infection. The qRT-PCR in infected silks showed that glucanase, lipid transfer, xylanase inhibitor, PR1 and 26S proteosome transcripts had higher expression ratios in the susceptible line compared to the resistant one in response to fungal infection. Through this study, a global view of differential genes expressed and metabolites concentration during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.
2013-08-17 | GSE40288 | GEO
Project description:Transcription profiling by high throughput sequencing of resistant and susceptible maize genotypes after inoculation with Fusarium verticillioides
2019-05-14 | E-MTAB-4219 | ExpressionAtlas
Project description:Fusarium graminearum gene expression profiles were compared during an early infection time course (1, 2, and 4d post-inoculation) of three monocot hosts (wheat heads, barley heads, and maize developing kernels).
2015-09-30 | GSE37886 | GEO
Project description:Maize kernels are susceptible to infection by the opportunistic pathogen Aspergillus flavus. Infection results in reduction of grain quality and contamination of kernels with the highly carcinogenic mycotoxin, aflatoxin. To understand host response to infection by the fungus, transcription of approximately 9,000 maize genes were monitored during the host-pathogen interaction with a custom-designed Affymetrix GeneChip® DNA array. More than 1,000 maize genes were found differentially expressed at a fold change of 2 or greater. This included the up regulation of defense-related genes and signaling pathways. Transcriptional changes also were observed in primary metabolism genes. Starch biosynthetic genes were down regulated during infection, while genes encoding maize hydrolytic enzymes, presumably involved in the degradation of host reserves, were up regulated. These data indicate that infection of the maize kernel A. flavus induced metabolic changes in the kernel, including the production of a defense response, as well as a disruption in kernel development. Maize kernels were mock inoculated at the blister (R2) or dough (R4) stage or inoculated with A. flavus at the blister (R2), milk (R3), dough (R4), or dent (R5) stage, and harvested 4 days later. Each treatment consisted of three biological replications. For each biological replication, 8 kernels were ground and RNA was isolated and further processed.
2014-05-14 | E-GEOD-57629 | biostudies-arrayexpress
Project description:We used microarrays to characterize maize kernels in which the expression of two endosperm-specific type-A response regulators, ZmTCRR1 and ZmTCRR2, was downregulated. Target gene downregulation was achieved by introducing a double RNAi construct in the maize genome. We compared the transcriptome of sibling kernels harvested 11 days after pollination from three independent transgenic events. Seeds were first sorted into transgenic and non-transgenic classes, and then the downregulation of the target genes was verified in the transgenic kernels by real-time PCR and Western blot.
2022-05-01 | E-MTAB-11424 | biostudies-arrayexpress
Project description:A next-generation RNA-sequencing approach was used for the first time to study transcriptional changes associated with Fusarium verticillioides inoculation in resistant CO441 and susceptible CO354 maize genotypes at 72 hours post inoculation.
2014-10-06 | E-MTAB-4219 | biostudies-arrayexpress
Project description:eQTL analysis of 5 DAP Zea mays (maize) kernels
2020-04-16 | E-GEOD-110315 | ExpressionAtlas
Project description:In the study, expression profiles in wild type and a fcc1 mutant were compared to identify genes with patterns of expression similar to the FUM genes. DNA microarrays were constructed with ESTs from cDNA libraries obtained from the fcc1 mutant and wild-type strain of F. verticillioides grown on maize kernels . By microarray methodology, gene expression was measured in the fcc1 mutant and the wild type during growth on maize and in a synthetic medium at pH 3 and 8. The results of the analysis provide potential candidate genes for future studies to determine their functional roles in fumonisin biosynthesis. Keywords: other
2004-01-02 | GSE938 | GEO