Transcription profiling of human primary T cell culture and T cell response to oxidative stress

ABSTRACT: Little is known about the global transcriptional program underlying T-cell activation and T-cell response to oxidative stress. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of human T-cell activation and T-cell response to H2O2 stress. The goal of this study was to identify genes involved in the various facets of human T-cell activation and T-cell response to H2O2 stress. Experiment Overall Design: T cells isolated from peripheral blood were cultured with CD3, CD28, and IL-2 to induce T-cell activaiton. Samples were splited into two parts before the cutlure started, one treated with 25 uM H2O2 for 10 minutes, the other intact. At each timepoint, cells were harvested and frozen for RNA isolation. Three biological replicate experiments were analyzed and appr

ORGANISM(S): Homo sapiens

SUBMITTER: Eleftherios Papoutsakis 

PROVIDER: E-GEOD-6607 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress