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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA Sequencing of default, melanocyte biased and enteric human neural crest populations (NC) and neuroectoderm (CNS)

ABSTRACT: We have generated expression profiles of three different neural crest populations from human embryonic stem cells. These profiles were compared to a neuroectoderm population. We find that the neural crest populations are separable and distinct. All cell types were differentiated from human embryonic stem cells. Neural crest populations were sorted on day 12 for CD49. The neuroectoderm cells were unsorted and harvested on day 12 of differentiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Lorenz Studer

PROVIDER: E-GEOD-66148 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease.

Fattahi Faranak F Steinbeck Julius A JA Kriks Sonja S Tchieu Jason J Zimmer Bastian B Kishinevsky Sarah S Zeltner Nadja N Mica Yvonne Y El-Nachef Wael W Zhao Huiyong H de Stanchina Elisa E Gershon Michael D MD Grikscheit Tracy C TC Chen Shuibing S Studer Lorenz L

Nature 20160210 7592

The enteric nervous system (ENS) is the largest component of the autonomic nervous system, with neuron numbers surpassing those present in the spinal cord. The ENS has been called the 'second brain' given its autonomy, remarkable neurotransmitter diversity and complex cytoarchitecture. Defects in ENS development are responsible for many human disorders including Hirschsprung disease (HSCR). HSCR is caused by the developmental failure of ENS progenitors to migrate into the gastrointestinal tract, ...[more]

PMID: 26863197

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