Chemical modifications mark alternatively spliced and actively degrading messenger RNAs in Arabidopsis and humans

ABSTRACT: Post-transcriptional chemical modification of RNA bases is a widespread and physiologically relevant regulator of RNA maturation, stability, and function. While modifications are best characterized in short, noncoding RNAs such as transfer RNAs (tRNAs), growing evidence indicates that messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) are likewise modified. Here, we apply our High-throughput Annotation of Modified Ribonucleotides (HAMR) pipeline to identify and classify modifications that affect Watson-Crick base-pairing at three different levels of the human and Arabidopsis thaliana transcriptomes (polyadenylated, small, and degrading RNAs). We find modifications primarily within actively degrading mRNAs and lncRNAs, suggesting they can act as a potent signal for RNA turnover. Addit

ORGANISM(S): Homo sapiens

SUBMITTER: Lee Vandivier 

PROVIDER: E-GEOD-66224 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress