Effect of decitabine, carboplatin and their combination on gene expression in the YB5 colon cancer cell line.

ABSTRACT: YB5 cell line is a clonal derivative of the SW48 colon cancer cell line that contains a single copy of a green fluorescent protein (GFP) reporter silenced by CpG methylation at its CMV promoter. We treated the YB5 cells with decitabine (5-aza-2’-deoxycytidine), carboplatin and the combination of decitabine plus carboplatin and analyzed the effects of the drugs on gene expression. YB5 cells in the early log phase of growth were seeded at 3.5 million in 20 ml of culture medium in 10 cm diameter tissue culture plates at day 0. The cells were treated with 20 microliters of decitabine (final concentration 25 nM), carboplatin (final concentration 20 uM) or both at days 1, 2, 3 and 4. Control cells were treated with 20 microliters of diluent (DMSO). The cells were harvested at day 6. Total RNA w

ORGANISM(S): Homo sapiens

SUBMITTER: Jaroslav Jelinek 

PROVIDER: E-GEOD-66279 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress