WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

ABSTRACT: The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. We identified by ChIP-seq >1680 high-confidence WRKY33 binding sites associated with 1576 genes within the Arabidopsis genome, with all of them being dependent on rapid activation of WRKY33 expression by Botrytis cinerea strain 2100. Genome-wide transcriptional analysis defined 318 genes as direct functional targets at 14 h post inoculation. Comparison between resistant wild-type Columbia-0 and susceptible wrky33 mutant plants revealed that expression of 75% of all WRKY33 regulated targets were down-regulated upon infection, indicating that WRKY33 predominately acts as a repressor. However, WRKY33 appears to possess dual functionality acting either as a repressor or as an activator in

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Barbara Kracher 

PROVIDER: E-GEOD-66289 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress