Transcription profiling of human HED293 cells with GFP constructs at different genomic positions are expressed corresponding to the activity of the domains of integration

ABSTRACT: Transcription factor complexes bind to regulatory sequences of genes, providing a system of individual expression regulation. Targets of distinct transcription factors usually map throughout the genome, without clustering. Nevertheless, highly and weakly expressed genes do cluster in separate chromosomal domains with an average size of 80 to 90 genes. We therefore asked whether, besides transcription factors, an additional level of gene expression regulation exists that acts on chromosomal domains. Here we show that identical green fluorescent protein (GFP) reporter constructs integrated at 90 different chromosomal positions determined by sequencing, obtain expression levels that correspond to the activity of the domains of integration. These domains are about 80 genes long and can exert

ORGANISM(S): Homo sapiens

SUBMITTER: Rogier Versteeg 

PROVIDER: E-GEOD-6629 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress