Small RNA sequencing of transgenes silencing by paramutation in C. elegans

ABSTRACT: In the nematode Caenorhabditis elegans, different small RNA-dependent gene silencing mechanisms act in the germline to initiate transgenerational gene silencing. Piwi-interacting RNAs (piRNAs) can initiate transposon and gene silencing by acting upstream of endogenous short interfering RNAs (siRNAs), which engage a nuclear RNA interference (RNAi) pathway to trigger transcriptional gene silencing. Once gene silencing has been established, it can be stably maintained over multiple generations without the requirement of the initial trigger and is also referred to as RNAe or paramutation. This heritable silencing depends on the integrity of the nuclear RNAi pathway. However, the exact mechanism by which silencing is maintained across generations is not understood.Here we demonstrate that silen

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Peter Sarkies 

PROVIDER: E-GEOD-66344 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress