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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Rat DNA methylation in the Nucleus Accumbens in Incubation of Cocaine Craving and treatments with DNA methylation modifying drugs

ABSTRACT: DNA methylation profiling of nucleus Accumbens of rats that self administered cocaine, were subjected to 30 withdrawal days, were treated with aCSF, RG108 or SAM and were subjected to extinction tests. The groups consist of: 1. Rats that self-administered cocaine for 10 days and that were subjected to a withdrawal period of 30 days, were injected in the nucleus accumbens with aCSF and were subjected to an extinction test for assessment of cue-induced cocaine-seeking behavior (aCSF) 2. Rats that self-administered cocaine for 10 days and that were subjected to a withdrawal period of 30 days, were injected in the nucleus accumbens with RG108 and were subjected to an extinction test for assessment of cue-induced cocaine-seeking behavior (RG108) 3. Rats that self-administered cocaine for 10 days and that were subjected to a withdrawal period of 30 days, were injected in the nucleus accumbens with SAM and were subjected to an extinction test for assessment of cue-induced cocaine-seeking behavior (SAM)

ORGANISM(S): Rattus norvegicus

SUBMITTER: Matthew Suderman

PROVIDER: E-GEOD-66347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rat gene expression in the Nucleus Accumbens in Incubation of Cocaine Craving

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2015-02-27 | E-GEOD-66349 | biostudies-arrayexpress

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2014-07-22 | PXD000990 | Pride

S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice

Project description:The aim of the study was to investigate whether environmental factors like S-adenosylmethionine (SAM) via affecting epigenome could alter cocaine-induced gene expression and locomotor sensitization in mice. Using mouse nucleus accumbens (NAc) tissue, whole-genome gene expression profiling revealed that repeated SAM treatment affected a limited number of genes, but significantly modified cocaine-induced gene expression by blunting nonspecifically the cocaine response. At the gene level, we discovered that SAM modulated cocaine-induced DNA methylation by inhibiting both promoter-associated CpG-island hyper- and hypomethylation in the NAc but not in the reference tissue cerebellum. Total RNA was extracted from the mouse nucleus accumbens (NAc) tissue. Two tissues were combined to a sample, 4 samples per group used. RNA quality and quantity were assessed using the Nano-Drop -1000 spectrophotometer and the Agilent 2100 Bioanalyzer.

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