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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: dan liu

PROVIDER: E-GEOD-66382 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ATF4 plays a pivotal role in the development of functional hematopoietic stem cells in mouse fetal liver.

Zhao Yunze Y Zhou Jie J Liu Dan D Dong Fang F Cheng Hui H Wang Weili W Pang Yakun Y Wang Yajie Y Mu Xiaohuan X Ni Yanli Y Li Zhuan Z Xu Huiyu H Hao Sha S Wang Xiaochen X Ma Shihui S Wang Qian-fei QF Xiao Guozhi G Yuan Weiping W Liu Bing B Cheng Tao T

Blood 20150917 21

The fetal liver (FL) serves as a predominant site for expansion of functional hematopoietic stem cells (HSCs) during mouse embryogenesis. However, the mechanisms for HSC development in FL remain poorly understood. In this study, we demonstrate that deletion of activating transcription factor 4 (ATF4) significantly impaired hematopoietic development and reduced HSC self-renewal in FL. In contrast, generation of the first HSC population in the aorta-gonad-mesonephros region was not affected. The m ...[more]

PMID: 26384355

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ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment (array)

Project description:In this study, we demonstrated that deletion of the activating transcription factor 4 (ATF4) resulted in severely impaired HSC expansion in the fetal liver at E12.5 and E15.5. In contrast, generation of the first HSC population in the aorta-gonad-mesonephros region at E11.5 was not significantly affected. Furthermore, the HSC-supporting ability of both endothelial and stromal cells in fetal liver was significantly compromised in the absence of ATF4. Gene profiling using RNA-seq revealed down-regulated expression of a panel of cytokines in ATF4-/- stromal cells, including angiopoietin-like protein 3 (Angptl3) and vascular endothelial growth factor-A (VEGFA). To investigate the molecular pathways of ATF4 in the stromal cells and LSK cells in the fetal liver.

2015-11-20 | E-GEOD-66381 | biostudies-arrayexpress

ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment (RNA-Seq)

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ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-11-20 | GSE66382 | GEO

ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment (array)

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ATF4 governs functional expansion of hematopoietic stem cells partially via Angptl3 in the fetal liver microenvironment (RNA-Seq)

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MicroRNA-203 represses selection and expansion of oncogenic HRas transformed tumor initiating cells [array]

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Expression comparison of mutant EGFR-driven gliomas with relapse tumors upon mutant EGFR ablation

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2015-01-08 | E-GEOD-64751 | biostudies-arrayexpress

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