De Novo Assembly and Characterization of Stress Transcriptome in a salinity-Tolerant Variety CS52 of Brassica juncea

ABSTRACT: Oilseed mustard, Brassica juncea, exhibits high levels of genetic variability for salinity tolerance. To obtain the global view of transcriptome and investigate the molecular basis of salinity tolerance in a salt-tolerant variety CS52 of B. juncea, we performed transcriptome sequencing of control and salt-stressed seedlings. De novo assembly of 184 million high-quality paired-end reads yielded 42,327 unique transcripts longer than 300 bp with RPKM ≥1. When compared with non-redundant proteins, we could annotate 67% unigenes obtained in our study. Based on the mapping to expressed sequence tags (ESTs), 52.6% unigenes are novel compared to EST data available for B. juncea and constituent genomes. Differential expression analysis revealed altered expression of 1469 unigenes in response to sal

ORGANISM(S): Brassica juncea

SUBMITTER: Ashwani Pareek 

PROVIDER: E-GEOD-66389 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress