The landscape of accessible chromatin in mammalian pre-implantation embryos

ABSTRACT: In mammals, extensive chromatin reorganization is essential for reprogramming terminally committed gametes to a totipotent state during preimplantation development. However, the global chromatin landscape and its dynamics in this period remain unexplored. Here we report a genome-wide map of accessible chromatin in mouse preimplantation embryos using an improved assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) approach with CRISPR/Cas9-assisted mitochondrial DNA depletion. We show that despite extensive parental asymmetry in DNA methylomes, the chromatin accessibility between the parental genomes is globally comparable after major zygotic genome activation (ZGA). Accessible chromatin in early embryos is widely shaped by transposable elements and overlaps

ORGANISM(S): Mus musculus

SUBMITTER: Wei Xie 

PROVIDER: E-GEOD-66390 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress