SW1 mutant vs Shewanella piezotolerans WP3 wild-type strain
Ontology highlight
ABSTRACT: The prophage and RF DNA of SW1 were removed from WP3 to construct the phage-free strain. The two strains were cultured at 20MPa and 4M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The hns gene was deleted from WP3 genome to construct the WP3M-NM-^Thns strain. The two strains were cultured at 20M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The hns gene was deleted from WP3 genome to construct the WP3M-NM-^Thns strain. The two strains were cultured at 4M-BM-0C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The cAMP binding domain of crp gene was deleted from WP3 genome to construct the WP3?crpD1 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:The DNA binding domain of crp gene was deleted from WP3 genome to construct the WP3?crpD2 strain. The two strains were cultured at 20°C and the transcriptional profiles were compared. Three biological replicates sample and 6 technical replicates for each experiment
Project description:Comparing the gene expression profiling of HDGF-silenced RD-ES cells and control RD-ES cells to identify genes regulated by HDGF in RD-ES cells. Keywords: expression analysis Control RD-ES cells and HDGF-silenced RD-ES cells were profiled on 22K Human Genome Array
Project description:Expression changes in silkworm integuments after JH analogue (JHA) methoprene treatment. Two-condition experiments, namely JH analogue (JHA) methoprene treatment (Test) and acetone treatment (Control). Time course: 12 hours after treatment (Hat), 24 Hat, 36 Hat, and 48 Hat.
Project description:Microsporidia have attracted much attention because they infect a variety of species ranging from protists to mammals, including immunocompromised patients with AIDS or cancer. Aside from the study on Nosema ceranae, few works have focused on elucidating the mechanism in host response to microsporidia infection. Nosema bombycis is a pathogen of silkworm pM-CM-)brine that causes great economic losses to the silkworm industry. Detailed understanding of the host (Bombyx mori) response to infection by N. bombycis is helpful for prevention of this disease. The 23 K silkworm genome array was used to investigate host responses (i.e., Bombyx mori) occurring at 2, 4, 6 and 8 d post-infection by Nosema bombycis.We focused on elucidating the mechanism of the host response to microsporidia infection, especially for the investigation of host immune response . The third instar molted silkworm larvae were in oral infected by Nosema bombycis. In order to known the silkworm host response to Nosema bombycis infection at different time points, samples of infected larvae (i.e., the treatment set) and uninfected larvae (i.e., the control set) were collected at 2, 4, 6 and 8 dpi for RNA extraction and array hybridization. The obtained data were usd to investigate on the interplay of the genome-wide expression profile of hosts.
Project description:Transcriptional characteristics of genes in the midgut of domestic silkworms after 24 h exposure to phoxim through whole-genome oligonucleotide microarray. Transcription profiling experiments, phoxim-treated midgut (samples) were analyzed. Dual-channel experiments, with test samples labeled by Cy5 and control samples labeled by Cy3. Three Biological replicate. No dye-swaps.
Project description:Transcriptional profiling of silkworm BmN4-SID1 cells comparing the test of BmSoxE knockdown with the control of EGFP Knockdown. Two-condition experiment, BmSoxE knockdown vs EGFP Knockdown. Biological replicates: 3. One replicate per array.