Regulation of gene expression by WAC/RNF20/RNF40 in HEK293A cells

ABSTRACT: WAC is a known positive regulator of (macro)autophagy. WAC also forms a complex with RNF20/RNF40 to promote H2B monoubiquitination and hence to affect transcriptional regulation. This study addresses whether the WAC/RNF20/RNF40 complex regulates autophagy through effects on gene expression. WAC, RNF20 and RNF40 were knocked-down using pools of siRNAs in HEK293A cells. Each knockdown was in triplicate and the control was RISCfree siRNA. mRNA expression profiles were investigated using an Illumina HT12v4 Bead Array.

ORGANISM(S): Homo sapiens

SUBMITTER: Sharon Tooze 

PROVIDER: E-GEOD-66475 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress