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Biochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA

ABSTRACT: Biochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA. Nuclei from three biological replicates were isolated by detergent lysis, fractionated, then three chromatin and three soluble RNA samples were converted to cDNA using Illumina TruSeq stranded protocol, and sequenced on Illumina HiSeq2000

ORGANISM(S): Homo sapiens

SUBMITTER: Alex Ruthenburg

PROVIDER: E-GEOD-66478 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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