Ectoderm specification of H1 human embryonic stem cells

ABSTRACT: In recent years, several studies have shed light into the processes that regulate epidermal specification and homeostasis. We previously showed that a broad-spectrum γ–secretase inhibitor DAPT promoted early keratinocyte specification in human embryonic stem cells triggered to undergo ectoderm specification. Here, we show that DAPT accelerates human embryonic stem cell differentiation and induces expression of the ectoderm protein AP2. Furthermore, we utilize RNA sequencing to identify several candidate regulators of ectoderm specification including those involved in epithelial and epidermal development in human embryonic stem cells. Genes associated with transcriptional regulation and growth factor activity are significantly enriched upon DAPT treatment during specification of human embry

ORGANISM(S): Homo sapiens

SUBMITTER: Valerie horsley 

PROVIDER: E-GEOD-66506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress