Single-Cell RNA-seq Defines the Three Cell Lineages of the Human Blastocyst

ABSTRACT: Despite morphological similarities, relatively little is known about conserved developmental processes in human and mouse pre-implantation embryos. Here we provide the first comprehensive single-cell RNA-sequencing comparison of human and mouse embryos from the zygotic to the blastocyst stage. We establish a robust computational pipeline allowing us to elucidate human-specific transcriptional Programs. Importantly, we validate our RNA-sequencing findings by Immunofluorescence analysis, which further reveals differences in human and mouseembryo gene expression. For example, although key trophectoderm factors Id2, Elf5, Eomes and Tcfap2c/Ap2? are exclusively localized to this lineage in the mouse, the human orthologues are either absent or expressed in alternative lineages. Importantl

ORGANISM(S): Homo sapiens

SUBMITTER: paul blakeley 

PROVIDER: E-GEOD-66507 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress