GAS5 controls Nodal autocrine in Human Embryonic Stem Cells Self-Renewal through competing endogenous mechanism

ABSTRACT: Long non-coding RNAs (lncRNAs) are recently characterized players that are involved in the regulatory circuitry of self-renewal in human embryonic stem cells (hESCs). However, the specific roles of lncRNAs in this circuitry are poorly understood. Here, we determined that growth-arrest-specific transcript 5 (GAS5), which is a known tumor suppressor and growth arrest gene, is abundantly expressed in the cytoplasm of hESCs and essential for hESC self-renewal. GAS5 depletion in hESCs significantly impaired their pluripotency and self-renewal ability, whereas GAS5 overexpression in hESCs accelerated the cell cycle, enhanced their colony formation ability and increased pluripotency marker expression. By RNA sequencing and bioinformatics analysis, we determined that GAS5 activates NODAL-SMAD2/3 s

ORGANISM(S): Homo sapiens

SUBMITTER: Chen Xu 

PROVIDER: E-GEOD-66993 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress