KRAS-Dependent Sorting of miRNAs and other small RNAs to secreted vesicles

ABSTRACT: Although long thought to act cell autonomously, mutant KRAS colorectal cancer (CRC) cells release protein-laden exosomes that can alter the tumor microenvironment. We have previously shown that mutant KRAS induces EGFR-ligand trafficking to exosomes and drastically alters exosomal protein contents, leading to activities that contribute to neoplastic growth. We have performed small library RNAseq analysis on cells and matched exosomes from isogenic CRC cell lines differing only in KRAS status to determine whether mutant KRAS regulates the composition of secreted small RNAs. Exosomal small RNA profiles were distinct from cellular profiles, with principle component analysis showing clusters of mutant KRAS cell-derived exosomes distinct from wild type KRAS cell-derived exosomes. Secreted RNA

ORGANISM(S): Homo sapiens

SUBMITTER: Yongchao Dou 

PROVIDER: E-GEOD-67004 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress