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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Enrichment of H3K9me2 on unsynapsed chromatin in C. elegans does not target de novo sites [RNA-Seq]

ABSTRACT: We sequenced mRNA isolated from adult hermaphrodite met-2;him-8 and him-8 gonads in order to determine the gene expression profile of germ cells with or without H3K9me2. Examination of mRNA profiles in nematode gonad of met-2;him-8 (without H3K9me2) and him-8 (with H3K9me2)

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Eleanor Maine

PROVIDER: E-GEOD-67029 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Enrichment of H3K9me2 on Unsynapsed Chromatin in Caenorhabditis elegans Does Not Target de Novo Sites.

Guo Yiqing Y Yang Bing B Li Yini Y Xu Xia X Maine Eleanor M EM

G3 (Bethesda, Md.) 20150708 9

Many organisms alter the chromatin state of unsynapsed chromosomes during meiotic prophase, a phenomenon hypothesized to function in maintaining germline integrity. In Caenorhabditis elegans, histone H3 lysine 9 dimethylation (H3K9me2) is detected by immunolabeling as enriched on unsynapsed meiotic chromosomes. Loss of the SET domain protein, MET-2, greatly reduces H3K9me2 abundance and results in germline mortality. Here, we used him-8 mutations to disable X chromosome synapsis and performed a ...[more]

PMID: 26156747

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Enrichment of H3K9me2 on unsynapsed chromatin in C. elegans does not target de novo sites

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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