A novel phosphate-starvation response in fission yeast requires the endocytic function of Myosin I (ChIP-seq)

ABSTRACT: S. pombe Myosin I (MyoI) plays a key role in cytoskeletal functions, in particular it is part of the endocytotic machinery. Here we show a novel role for MyoI in regulating the response to low phosphate stress. Deletion of MyoI led to an increased proliferation in low phosphate media when compared to a control strain; mutations in other proteins involved in the early steps of endocytosis, such as ARP2 and Sla2/END4, led to the same phenotype. We show that once cells are deprived of phosphate they undergo clear morphological changes, slow down growth but retain viability. This is reminiscent of a quiescence response, and it is completely abolished when MyoI is absent. Transcriptomic analysis revealed a wide perturbation of gene expression upon phosphate starvation, with rapid induction of s

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Felix Krueger 

PROVIDER: E-GEOD-67105 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress