KDM5 Lysine Demethylases Are Involved in Maintenance of 3'UTR Length [RNA-Seq]

ABSTRACT: We discovered that the Saccharomyces cerevisiae lysine demethylase, Jhd2 (also known as KDM5), recruits 3'UTR processing machinery and promotes alteration of 3'UTR length in a demethylase-dependent manner. Interaction of Jhd2 with both chromatin and RNA suggests that Jhd2 affects selection of polyadenylation sites through a transcription-coupled mechanism. For 3'READs analysis, wild-type yeast or yeast with JHD2 deleted were grown to mid-log phase and RNA was extracted as mentioned above. cDNA libraries enriched for 3'UTRs were prepared as previously published and as noted in Figure 4a [28]. Samples were then subjected to RNA sequencing on the Illumina Hiseq 2000 using 50bp single end reads. Data was analyzed as previously [Hoque M] with the following modifications: The adapter sequen

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Valentina Greco 

PROVIDER: E-GEOD-67163 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress