Transcription profiling of mouse liver treated with phenobarbital and fed a high cholesterol diet an

ABSTRACT: High cholesterol diet and xenobiotic treatment induce changes in cholesterol homeostasis and drug metabolism. Mice were either 7 days on high cholesterol diet or were treated with phenobarbital. Liver samples were anayzed using Affymetrix GeneChip MOE430A. Experiment Overall Design: One group of mice was treated 50 mg/kg of phenobarbital in vehicle (5% DMSO in corn oil). Untreated group was injected vehicle only. Third group was 7 days on 1% (w/w) cholesterol diet prior vehicle treatment. After 10 hours animals were sacrificed and total RNA was isolated from liver. Total RNA from two animals was pooled resulting in 4 pools per group. These pools were analyzed using Affymetrix GeneChip MOE430A.

ORGANISM(S): Mus musculus

SUBMITTER: Peter Juvan 

PROVIDER: E-GEOD-6721 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress