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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO1]

ABSTRACT: Analysis of gene expression in ovarian cancer cells with and without overexpression of the miRNA mir-124 which will provide data about genes possibly directly regulated by miR-124. Also, analysis of gene expression in ovarian cancer cells with and without knockdown of the homeodomain transcription factor SIX4 (a target of miR-124) which will provide data about genes possibly directly regulated by SIX4 Total RNA was isolated from PEO1 cells transfected with miRNA mimic negative control (miNC), miR-124 mimic, siRNA negative control (siNC) oligo, or siRNA targeting the SIX4 gene (siSIX4) 72 hours post-transfection

ORGANISM(S): Homo sapiens

SUBMITTER: Michael White

PROVIDER: E-GEOD-67327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO4]

Project description:Analysis of gene expression in ovarian cancer cells with and without overexpression of the miRNA mir-124 which will provide data about genes possibly directly regulated by miR-124. Also, analysis of gene expression in ovarian cancer cells with and without knockdown of the homeodomain transcription factor SIX4 (a target of miR-124) which will provide data about genes possibly directly regulated by SIX4 Total RNA was isolated from PEO4 cells transfected with miRNA mimic negative control (miNC), miR-124 mimic, siRNA negative control (siNC) oligo, or siRNA targeting the SIX4 gene (siSIX4) 72 hours post-transfection

2015-03-27 | E-GEOD-67328 | biostudies-arrayexpress

microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO4]

2015-03-27 | GSE67328 | GEO

microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO1]

2015-03-27 | GSE67327 | GEO

RNA-seq analysis of NSC-34 mouse motor neuron-like cells treated with Negative Control siRNA#2 (siNC#2), miR-125b-5p mimic, mmu-miR-124-3p mimic and siRNA (-) control

Project description:RNA-seq analysis of NSC-34 mouse motor neuron-like cells treated with Negative Control siRNA#2 (siNC#2), miR-125b-5p mimic, mmu-miR-124-3p mimic and siRNA (-) control

| PRJNA517108 | ENA

microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-03-27 | E-GEOD-67330 | biostudies-arrayexpress

Network-based integration of multi-omics data identifies the determinants of miR-491-5p effects

Project description:The identification of miRNAs’ targets and associated regulatory networks might allow the definition of new strategies using drugs whose association might mimic a given miRNA’s effects. Based on this assumption our group devised a multi-omics approach in an attempt to precisely characterize miRNAs’ effects. We combined the analysis of miR-491-5p direct targets, and effects at the transcriptomic and proteomic levels. We thus constructed an interaction network which enlightened highly connected nodes, being either direct or indirect targets of miR-491-5p effects: the already known EGFR and BCL2L1, but also EP300, CTNNB1 and several small-GTPases. By using different combinations of specific inhibitors of these nodes, we could greatly enhance their respective cytotoxicity and mimic miR-491-5p-induced phenotype. Our methodology thus constitutes an interesting strategy to comprehensively study the effects of a given miRNA. Also, we identified targets for which pharmacological inhibitors are already available for a clinical use, or in clinical trial phases. This study might thus enable innovative therapeutic options for ovarian cancer, which remains the first cause of death from gynecological malignancies in developed countries.

2021-09-09 | PXD022131 | Pride

Gene Expression Profiling of siRNA Knock-Down of Six1, Six4 and Myogenin in C2C12

2010-07-05 | GSE19967 | GEO

Gene Expression Profiling of siRNA Knock-Down of Six1, Six4 and Myogenin in C2C12

Project description:Six1, Six4 and Myogenin are transcription factors that are known to be required for skeletal myogenesis. Currently, very little is known about the genes targeted by Six1 and Six4. Gene expression profiling when one or both transcription factors were knock-down by siRNA was performed to identify genes affected by their absence. We also hypothesized that Six1 and Six4 can work in cooperation with the myogenic regulatory factor (MRFs) family of transcription factors, such as Myogenin. Therefore, we performed the same type of experiment where the myogenin was knocked-down by siRNA to identify genes that are possibly regulated by the Six1 or Six4 in conjunction with Myogenin. C2C12 Myoblasts were transfected with siRNA against Six1, Six4, Six1 with Six4, Myogenin, or control 24h before start of differentiation. The cells were allowed to differentiate in differentiation medium for 24h and were harvested for gene expression profiling. Four replicates per siRNA were performed.

2010-07-30 | E-GEOD-19967 | biostudies-arrayexpress

Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-124 mimic

Project description:Expression data from Huh7.5 hepatoma cells transfected with 100 nM miR-124 mimic

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2011-05-30 | E-GEOD-28456 | biostudies-arrayexpress

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