A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation

ABSTRACT: The inactive X chromosome (Xi) serves as a model to understand gene silencing on a global scale. Here, we perform identification of direct RNA interacting proteins? (iDRiP) to isolate a comprehensive protein interactome for Xist, an RNA required for Xi silencing. We discover multiple classes of interactors, including cohesins, condensins, topoisomerases, RNA helicases, chromatin remodelers and modifiers, which synergistically repress Xi transcription. Inhibiting two or three interactors destabilizes silencing. While Xist attracts some interactors, it repels architectural factors. Xist evicts cohesins from the Xi and directs an Xi-specific chromosome conformation. Upon deleting Xist, the Xi acquires the cohesin-binding and chromosomal architecture of the active X. Our study unveils many lay

ORGANISM(S): Mus musculus

SUBMITTER: John Froberg 

PROVIDER: E-GEOD-67516 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress