Protein phosphatase PP1-NIPP1 activates mesenchymal genes in HeLa cells

ABSTRACT: The deletion of the protein phosphatase-1 (PP1) regulator NIPP1 is embryonic lethal during gastrulation, hinting at a key role of PP1-NIPP1 in lineage specification. Consistent with this notion we show here that a mild, stable overexpression of NIPP1 in HeLa cells caused a massive induction of genes of the mesenchymal lineage, in particular smooth/cardiac-muscle and matrix markers. This reprogramming was associated with the formation of actin-based stress fibers and retracting filopodia, and a reduced proliferation potential. The NIPP1-induced mesenchymal transition required functional substrate and PP1-binding domains, suggesting that it involves the selective dephosphorylation of substrates of PP1-NIPP1. In total 16 samples were processed. Four dif

ORGANISM(S): Homo sapiens

SUBMITTER: Wouter Van Delm 

PROVIDER: E-GEOD-67558 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress