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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA expression in myeloid-derived suppressor cells (MDSCs) treated with or without whole ?-glucan particles (WGP)

ABSTRACT: Murine MDSCs isolated from the spleens of Lewis lung carcinoma mice were treated with or without WGP, and then miRNA array was used to analyze the differentailly expressed miRNAs. Murine MDSCs were isolated from the spleens of Lewis lung carcinoma tumor-bearing mice, and the sorted MDSCs were stimulated with or without 100 µg/ml WGP for 24 h. Then, the total RNA was extracted to perform miRNA array to analyze the differentially expressed miRNAs in MDSCs treated with or without WGP

ORGANISM(S): Mus musculus

SUBMITTER: Jie Tian

PROVIDER: E-GEOD-67578 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA-9 Regulates the Differentiation and Function of Myeloid-Derived Suppressor Cells via Targeting Runx1.

Tian Jie J Rui Ke K Tang Xinyi X Ma Jie J Wang Yungang Y Tian Xinyu X Zhang Yue Y Xu Huaxi H Lu Liwei L Wang Shengjun S

Journal of immunology (Baltimore, Md. : 1950) 20150619 3

Myeloid-derived suppressor cells (MDSCs) play a critical role in tumor-associated immunosuppression, thus affecting effective immunotherapies for cancers. However, the molecular mechanisms involved in regulating the differentiation and function of MDSCs remain largely unclear. In this study, we found that inhibition of microRNA (miR)-9 promoted the differentiation of MDSCs with significantly reduced immunosuppressive function whereas overexpression of miR-9 markedly enhanced the function of MDSC ...[more]

PMID: 26091714

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miRNA expression in myeloid-derived suppressor cells (MDSCs) treated with or without whole β-glucan particles (WGP)

Project description:Murine MDSCs isolated from the spleens of Lewis lung carcinoma mice were treated with or without WGP, and then miRNA array was used to analyze the differentailly expressed miRNAs.

2015-06-26 | GSE67578 | GEO

Analysis of differential miRNA expression profiling in Namalwa cells

Project description:To analyze the differential miRNA expression profiling in human lymphoma cells (Namalwa cell line) before and after treatment by DNA methylation inhibitor 5-aza-2'-deoxycytidine (Decitabine; 5-aza). Six total samples were analyzed, two groups, and triplicate for each group. The abbreviation of Con represents Namalwa cells without the treatment of 5-aza, and Add represents Namalwa cells after treatment by 5-aza for 3 days.

2013-12-27 | E-GEOD-53666 | biostudies-arrayexpress

Expression data from MDSCs at different time points during tumor progression

Project description:Myeloid-derived suppressor cells (MDSCs) are increased by tumor-derived factors and suppress anti-tumor immunity. MDSCs obtained at a late time point after tumor injection had stronger suppressive activity than MDSCs obtained at an early time point, as measured by T cell proliferation assays. To find factors in MDSCs that change during tumor growth, we analyzed gene expression profiles from MDSCs at different time points after tumor injection. We found that immune response-related genes were down-regulated, but pro-tumor function-related genes were up-regulated in both Mo-MDSCs and PMN-MDSCs at the late time point. Among differentially expressed genes, FK506 binding protein 51 (FKBP51), which is a member of the immunophilin protein family and plays a role in immunoregulation, was increased in the Mo- and PMN-MDSCs isolated from the late time points. Experiments using siRNA and a chemical inhibitor of FKBP51 revealed that FKBP51 contributes to the regulation of the suppressive function of MDSCs by increasing iNOS, ARG1, and ROS levels and enhancing NF-kappaB activity. Collectively, our data suggest that FKBP51 is a novel molecule that can be targeted to regulate the immunosuppressive function of MDSCs. To identify the factors that licensed MDSCs to be more suppressive as tumors grow, we analyzed gene expression profiles in the two subsets of MDSCs at different time points (3wks, 6wks) during tumor progression. CD11b+Ly-6C(high)Ly-6G(low) Mo-MDSCs and CD11b+Ly-6C(low)Ly-6G(high) PMN-MDSCs were sorted from pooled spleens of naÃ¯ve mice and Her-2/CT26 tumor-bearing mice. Total RNA was purified and gene expression was analyzed by the Affymetrix GeneChipÂ® Mouse Gene 1.0 ST Array.

2012-03-29 | E-GEOD-35398 | biostudies-arrayexpress

miRNAs expression profiling from mouse heart after Ang II (angiotensin II) (1500ng/kg/min) infusion for 3 days and 7 days

Project description:Angiotensin II stimulates fibroblast proliferation and substantially alters gene expression patterns leading to cardiac remodeling, but the mechanisms for such differences are unknown. MicroRNAs are a novel mechanism for gene expression regulation. Herein we tested the miRNAs patterns in mouse heart using microarray assay, and investigated their role in angiotensin II-induced cardiac remodeling. In this study, nine samples (three groups) from mouse were used to acquire the miRNA expression profiling and the function of the abudant miRNAs in the heart were analyzed by bioinformatic methods.

2015-06-05 | E-GEOD-69559 | biostudies-arrayexpress

The effects of herb-partitioned moxibustion on miRNA expression profiling in the colons of CD rats

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2015-01-31 | E-GEOD-65490 | biostudies-arrayexpress

Green Tea Polyphenol EGCG Attenuates MDSCs-mediated Immunosuppression through Canonical and Non-Canonical Pathways in a 4T1 Murine Breast Cancer Model

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2019-08-11 | GSE135685 | GEO

Triptolide alters the miRNA expression profiles in human hepatocellular carcinoma HepG2 cells

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2014-05-01 | E-GEOD-40037 | biostudies-arrayexpress

Formaldehyde exposure alters miRNAs expression profiles in olfactory bulb

Project description:Neurotoxicity of formaldehyde (FA) in the human health attracted intensive studies Long-term exposure to FA leads to learning and memory decline and is responsible for the multiple chemical sensitivity (MCS) or sick building syndrome (SBS). It was cleared that Formaldehyde impairs Learning and Memory in Hippocampal. however ,it is unclear if FA can disturb the olfactory bulb function miRNAs alterations were related to environmental chemical exposure. It was reported that FA inhale altered miRNAs expression in the nasal Epithelium and lung cells. In the present study, FA inhalation significatly alters miRNA expression profiles within olfactory bulb Eight week ICR male mouse. Mice were randomly assigned to three groups. The FA group exposed to 3ppm FA for six hours for consecutive 1 and 7days in a chamber which was described previously. The standard group was kept in same condition except the FA. After expose, both group mice were deeply anesthetized with isoflurane and decapitated. The OB was rapidly dissected

2015-08-04 | E-GEOD-59369 | biostudies-arrayexpress

Label free quantitativeproteomics of MDSCs

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microRNA Expression of mice corneal endothelia

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