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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of microRNA-27b target genes in breast cancer cell lines

ABSTRACT: To identify the microRNA-27b (miR-27b) target genes in luminal-type breast cancer cells, we performed the microarray analysis using miR-27b knockdown MCF7-luc cell line (MCF7-luc anti-miR-27b), miR-27b overexpressing MCF7-luc cell line (MCF7-luc miR-27b o.e.) and their contro cell line (MCF7-luc anti-NC). After establishing MCF7-luc anti-miR-27b, MCF7-luc miR-27b o.e. and MCF7-luc anti-NC using lentivirus vector, we performed the microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Ryou-u Takahashi

PROVIDER: E-GEOD-67631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss of microRNA-27b contributes to breast cancer stem cell generation by activating ENPP1.

Takahashi Ryou-u RU Miyazaki Hiroaki H Takeshita Fumitaka F Yamamoto Yusuke Y Minoura Kaho K Ono Makiko M Kodaira Makoto M Tamura Kenji K Mori Masaki M Ochiya Takahiro T

Nature communications 20150612

Cancer stem cells (CSCs) have been identified in various types of cancer; however, the mechanisms by which cells acquire CSC properties such as drug resistance and tumour seeding ability are not fully understood. Here, we identified microRNA-27b (miR-27b) as a key regulator for the generation of a side-population in breast cancer cells that showed CSC properties, and also found that the anti-type II diabetes (T2D) drug metformin reduced this side-population via miR-27b-mediated repression of ect ...[more]

PMID: 26065921

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Identification of microRNA-27b target genes in breast cancer cell lines

Project description:To identify the microRNA-27b (miR-27b) target genes in luminal-type breast cancer cells, we performed the microarray analysis using miR-27b knockdown MCF7-luc cell line (MCF7-luc anti-miR-27b), miR-27b overexpressing MCF7-luc cell line (MCF7-luc miR-27b o.e.) and their contro cell line (MCF7-luc anti-NC).

2015-06-18 | GSE67631 | GEO

MiR-23b/-27b Targets in Prostate Cancer Cell Lines

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MiR-23b/-27b Targets in Prostate Cancer Cell Lines

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miR-27b synergizes with anticancer drugs

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2015-09-22 | GSE73275 | GEO

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Heart mRNA profiles of TAC 8-weeks wild type (WT) and miR-27b-3p knockout mice

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2022-07-27 | GSE158191 | GEO

Analysis of gene expression influenced by miR-27b in Huh-7 cells.

Project description:To claryfy the genes regulated by miR-27b in human hepatic cells, whole human genome microarrays were employed to analyze gene expression.

2023-06-15 | GSE174075 | GEO

Effect of microRNA-27b-3p mimic treatment on human osteoarthritis fibroblast-like synoviocyte gene expression profiles

Project description:OBJECTIVE: To identify the changes in gene expression elicited by miR-27b-3p mimic transfection of human osteoarthritis (OA) primary fibroblast-like synoviocytes (FLS) obtained from patients undergoing knee arthroplasty. Cells were cultured, and treated for 48 h with miR-27b-3p mimic or Cel-miR-39-3p control mimic. Total RNA was then extracted, and mRNA libraries were prepared using Illumina's TruSEQ stranded total RNA library preparation kit, pooled together, and sequenced on Illumina's NextSeq550.

2022-08-30 | GSE152638 | GEO

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