Probing Xist RNA structure in cells using Targeted Structure-Seq

ABSTRACT: The long non-coding RNA (lncRNA) Xist is a master regulator of X-chromosome inactivation in mammalian cells. Models for how Xist and other lncRNAs function depend on thermodynamically stable secondary and higher-order structures that RNAs can form in the context of a cell. Probing accessible RNA bases can provide data to build models of RNA conformation that provide insight into RNA function, molecular evolution, and modularity. To study the structure of Xist in cells, we built upon recent advances in RNA secondary structure mapping and modeling to develop Targeted Structure-Seq, which combines chemical probing of RNA structure in cells with target-specific massively parallel sequencing. By enriching for signals from the RNA of interest, Targeted Structure-Seq achieves high coverage of the

ORGANISM(S): Mus musculus

SUBMITTER: Matthew Simon 

PROVIDER: E-GEOD-67667 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress