Spatially resolved, highly multiplexed RNA profiling in single cells

ABSTRACT: Knowledge of the expression profile and spatial landscape of the transcriptome in individual cells is essential for understanding the rich repertoire of cellular behaviors. Here we report multiplexed error-robust fluorescence in situ hybridization (MERFISH), a single-molecule imaging approach that allows the copy numbers and spatial localizations of thousands of RNA species to be determined in single cells. Using error-robust encoding schemes to combat single-molecule labeling and detection errors, we demonstrated the imaging of 100 – 1000 unique RNA species in hundreds of individual cells. Correlation analysis of the ~10^4 – 10^6 pairs of genes allowed us to constrain gene regulatory networks, predict novel functions for many unannotated genes, and identify distinct spatial distribution p

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaowei Zhuang 

PROVIDER: E-GEOD-67685 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress