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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse HDAC2 knockout myocardium

ABSTRACT: We used microarrays to detail the global programme of gene expression underlying cardiac development by HDAC2 and identified distinct classes of up-regulated and down-regulated genes during this process. Experiment Overall Design: Embryonic heart ventricles were dissected at E17.5 for RNA extraction and hybridization on Affymetrix microarrays. Wild type and HDAC2-deficient littermates were used. Tissue from 2-3 individual animals of identical genotype was pooled for each micro-array.

ORGANISM(S): Mus musculus

SUBMITTER: Jonathan Epstein

PROVIDER: E-GEOD-6770 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hdac2 regulates the cardiac hypertrophic response by modulating Gsk3 beta activity.

Trivedi Chinmay M CM Luo Yang Y Yin Zhan Z Zhang Maozhen M Zhu Wenting W Wang Tao T Floss Thomas T Goettlicher Martin M Noppinger Patricia Ruiz PR Wurst Wolfgang W Ferrari Victor A VA Abrams Charles S CS Gruber Peter J PJ Epstein Jonathan A JA

Nature medicine 20070218 3

In the adult heart, a variety of stresses induce re-expression of a fetal gene program in association with myocyte hypertrophy and heart failure. Here we show that histone deacetylase-2 (Hdac2) regulates expression of many fetal cardiac isoforms. Hdac2 deficiency or chemical histone deacetylase (HDAC) inhibition prevented the re-expression of fetal genes and attenuated cardiac hypertrophy in hearts exposed to hypertrophic stimuli. Resistance to hypertrophy was associated with increased expressio ...[more]

PMID: 17322895

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HOP homeobox (Hopx) and Histone deacetylase-2 (Hdac2) deficiency effect on the embryonic heart

Project description:Analysis of heart ventricles from Hopx, Hdac2, and both Hopx-Hdac2 deficient embryos at embryonic day E16.5. Results provide insight into the role of Hopx and Hdac2 in cardiac development. We used microarrays to detail the global programme of gene expression underlying cardiac development by Hopx and Hdac2 and identified distinct classes of up-regulated and down-regulated genes during this process. Mouse embryonic ventricles were selected at E16.5 for RNA extraction and hybridization on Affymetrix microarrays. We obtained three independent embryonic ventricles for WT, Hopx-null, Hdac2-null, and Hopx-Hdac2 double null genotypes.

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Gene Expression Data in HDAC2 KO Myocardium

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Expression data from primary MEF lacking either HDAC1, HDAC2 or both

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Quantitative proteomic SILAC analysis of enteroids deleted for Hdac1 or Hdac2

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Effect of HDAC2 siRNA on HCC

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Functions of HDAC1 and HDAC2 in Schwann cells during postnatal

Project description:The aim of our study is to determine the functions of histone deacetylases (HDACs) 1 and 2 in Schwann cells during postnatal development of the peripheral nervous system (PNS). Schwann cells are the myelinating glial cells of the PNS. At birth, mouse sciatic nerves mature in 2 subsequent phases: 1/ big caliber axons get sorted into a 1 to 1 relationship with Schwann cells, 2/ Schwann cells build a myelin sheath around sorted axons. In mice where both HDAC1 & HDAC2 have been specifically knocked out in Schwann cells, both phases are impaired. HDACs are chromatin remodeling enzymes, they can thus alter gene expression directly. We want to identify which genes controlled by HDAC1 and HDAC2 in Schwann cells are necessary for the maturation of sciatic nerves. Because HDAC1 and HDAC2 can compensate for each other loss to some extend, we will first analyze changes of gene expression in HDAC1/HDAC2 double KO animals. We expect to gain critical insights into the molecular mechanisms controlling Schwann cell differentiation and myelination. This knowledge is of key importance for the success of regenerative medicine in peripheral neuropathies, nerve tumors, and transplantation paradigms in non-regenerative CNS lesions and in large PNS injuries. 3 double knockout mutants for HDAC1 and HDAC2 and 3 control littermates were analyzed. Tissues analyzed: sciatic nerves of 2 day-old mouse pups

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