Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Deep sequencing of C. elegans microRNAs under starvation conditions

ABSTRACT: In nature, animals often face feast or famine conditions. We aimed to identify the miRNAs of Caenorhabditis elegans that changed their expression under starvation conditions in stage L4 larvae. Our results highlight 14 miRNAs that show differential expression in starved versus well-fed larvae. In particular, miRNAs of the miR-35-3p/miR-41-3p family were upregulated 6-20 fold upon starvation. We verified the upregulation of miR-35-3p with qPCR. Additionally, we showed that the expression of gld-1, important in ovogenesis, and a validated target of miR-35-3p, was downregulated when the expression of miR-35-3p was higher. This study represents a starting point for a more comprehensive understanding of the role of miRNAs during starvation in C. elegans. Illumina small RNA sequencing of starved and well-fed L4 worms.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Cei Abreu-Goodger

PROVIDER: E-GEOD-67711 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs.

Garcia-Segura Laura L Abreu-Goodger Cei C Hernandez-Mendoza Armando A Dimitrova Dinkova Tzvetanka D TD Padilla-Noriega Luis L Perez-Andrade Martha Elva ME Miranda-Rios Juan J

PloS one 20151110 11

MicroRNAs (miRNAs) are non-coding RNAs of ~22 nucleotides in length that regulate gene expression by interfering with the stability and translation of mRNAs. Their expression is regulated during development, under a wide variety of stress conditions and in several pathological processes. In nature, animals often face feast or famine conditions. We observed that subjecting early L4 larvae from Caenorhabditis elegans to a 12-hr starvation period produced worms that are thinner and shorter than wel ...[more]

PMID: 26554708

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Dataset Information

Deep sequencing of C. elegans microRNAs under starvation conditions

Publications

High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs.

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