Transcriptome of a mouse kidney cortical collecting duct cell line: effects of aldosterone and vasopressin

ABSTRACT: In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCD(cl4)) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with either aldosterone or vasopressin for 4 h. The transcriptomes in these three experimental conditions were determined by sequencing 169,721 transcript tags from the corresponding SAGE libraries. Limiting the analysis to tags that occurred twice or more in the data set, 14,654 different transcripts were identified, 3,642 of which do not match known mouse sequences. Statistical comparison (at P < 0.05 level) of the three SAGE libraries revealed 34 AITs (aldosterone-induced transcripts), 29 ARTs (a

ORGANISM(S): Mus musculus

SUBMITTER: Jean-Marc Elalouf 

PROVIDER: E-GEOD-678 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress