Project description:To test the effect of chronic water avoidance stress on spinal microRNA expression profile in male wistar rats. Lumbar spinal cord samples were freshly isolated from rats exposed to daily water avoidance stress for 10 consecutive days. Samples were collected 24 hours after the last stressors.
Project description:To test the effect of chronic water avoidance stress on spinal gene expression profile in male wistar rats. Lumbar spinal cord samples were freshly isolated from rats exposed to daily water avoidance stress for 10 consecutive days. Samples were collected 24 hours after the last stressors.
Project description:Identification of temporal variations in miRNA expression after spinal cord injury caused by thoracic (T8) moderate (200 Kdynes) contusion. Expression changes were analyzed 1, 3 and 7 days after injury and compared to expression of control (untreated) and sham (laminectony but no contusion) individuals. Included groups: control (untreated), 1 day after lesion, 3 days after lesion, 7 days after lesion, 1 days after SHAM cirugy, 3 days after SHAM cirugy, and 7 days after SHAM cirugy. Each of these 7 groups included 5 biological replicates.
Project description:The aim of our study is to identify miRNAs responsible for bone-cancer pain condition and their target mRNAs. We combined mRNA profiling with Affymetrix microarray and miRNA measurement with a qRT-PCR-based technique. Then, a cross-correlation of these data highlighted miRNA-mRNA pairs that were further characterized with functional experiments.
Project description:We used microarray gene expression analyses to unveil the mechanisms underlying NT-3-chitosan-induced spinal cord regeneration. Complete transaction of thoracic spinal cord at T7/8 was performed, with a 5 mm segment of the spinal cord being removed. The emptied space where either refilled with a 5mm chitosan tube loaded with or without NT-3 (NT-3 or empty tube, ET, respectively) or left alone (lesion control, LC). At 1, 3, 10, 20, 30, 60, 90days post surgery, animals were sacrificed, and 5mm segments of spinal cord at the lesion site, as well as immediately caudal or rostral to the lesion segment were collected labeled with R for rostral, C for caudal, and M for lesion site