The menu of features that define primary microRNAs and enable de novo design of microRNA genes

ABSTRACT: MicroRNAs (miRNAs) are small regulatory RNAs processed from stem-loop regions of primary transcripts (pri-miRNAs), with the choice of stem-loops for initial processing largely determining what becomes a miRNA. To identify sequence and structural features influencing this choice, we determined cleavage efficiencies of >50,000 variants of three human pri-miRNAs, focusing on the regions intractable to previous high-throughput analyses. Our analyses revealed a mismatched motif in the basal stem region, a preference for maintaining or improving base-pairing throughout the remainder of the stem, and a narrow stem-length preference of 35±1 base pairs. Incorporating these features with previously identified features, including three primary-sequence motifs, yielded a unifying model defining mammal

ORGANISM(S): synthetic construct

SUBMITTER: Wenwen Fang 

PROVIDER: E-GEOD-67937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress