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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Dual RNA-seq â sRNA profiling in various cell types

ABSTRACT: Bacteria possess many small noncoding RNAs whose regulatory roles in pathogenesis are little understood due to a paucity of macroscopic phenotypes in standard virulence assays. Here, we use a novel Dual RNA-seq approach for a single-step simultaneous RNA profiling in both pathogen and host to reveal molecular phenotypes of sRNAs during infection with Salmonella Typhimurium. We identify a new PhoP/Q-activated small RNA which upon bacterial internalization acts to temporally control the expression of both, invasion-associated effectors and virulence genes required for intracellular survival. This riboregulatory activity is shown to adjust the human response to replicating Salmonella, and have a pervasive impact on host RNA expression both inside and outside protein-coding regions including infection-specific alterations of an array of long noncoding RNAs. Our study provides a paradigm for a comprehensive RNA-based analysis of intracellular bacterial pathogens without their physical purification from a host and a new discovery route for hidden functions of pathogen genes. sRNA profiling in various cell types

ORGANISM(S): mixed sample: human cell line CaCo-2 and Salmonella Typhimurium SL1344

SUBMITTER: Konrad FÃ¶rstner

PROVIDER: E-GEOD-67947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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