Dual RNA-seq – sRNA profiling in various cell types

ABSTRACT: Bacteria possess many small noncoding RNAs whose regulatory roles in pathogenesis are little understood due to a paucity of macroscopic phenotypes in standard virulence assays. Here, we use a novel Dual RNA-seq approach for a single-step simultaneous RNA profiling in both pathogen and host to reveal molecular phenotypes of sRNAs during infection with Salmonella Typhimurium. We identify a new PhoP/Q-activated small RNA which upon bacterial internalization acts to temporally control the expression of both, invasion-associated effectors and virulence genes required for intracellular survival. This riboregulatory activity is shown to adjust the human response to replicating Salmonella, and have a pervasive impact on host RNA expression both inside and outside protein-coding regions including i

ORGANISM(S): mixed sample: human cell line CaCo-2 and Salmonella Typhimurium SL1344

SUBMITTER: Konrad Förstner 

PROVIDER: E-GEOD-67947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress