CSR-1 and P granules suppress sperm-specific transcription in the C. elegans germline

ABSTRACT: P granules in C. elegans are required for fertility and function to maintain germ cell identity and pluripotency.  Sterility in the absence of P granules is often accompanied by the mis-expression of soma-specific proteins and the initiation of somatic differentiation in germ cells.  To investigate whether this is caused by the accumulation of somatic transcripts, we performed mRNA-seq on dissected germlines with and without P granules.  Strikingly, we found that somatic transcripts do not increase in the young adult germline when P granules are impaired.  Instead, we found that impairing P granules causes sperm-specific mRNAs to become highly overexpressed.  This includes the accumulation of major sperm protein (MSP) transcripts in germ cells, a phenotype that is suppressed by feminizatio

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Dustin Updike 

PROVIDER: E-GEOD-67954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress