Widespread RNA Binding by Chromatin Associated Proteins

ABSTRACT: Recent evidence suggests that RNA interaction can regulate the activity and localization of DNA binding proteins, particularly chromatin-associated proteins. However, it is unknown if these observations are specialized instances for a few key RNAs and chromatin factors in specific contexts, or a general mechanism underlying the establishment of chromatin state and regulation of gene expression. Here, we introduce formaldehyde RNA ImmunoPrecipitation (fRIP-Seq), a sensitive method for cataloging RNA-protein interactions, to survey the RNA associated with a panel of 24 chromatin-associated and traditional RNA binding proteins. For each protein that reproducibly bound measurable quantities of bulk RNA (90% of the panel), we detected enrichment for hundreds to thousands of both noncoding and m

ORGANISM(S): Homo sapiens

SUBMITTER: David Kelley 

PROVIDER: E-GEOD-67963 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress